The correct version of the poster is available for downloading at. Function of patpase is to transport a variety of different compounds, like ions and phospholipids, across a membrane using atp hydrolysis for energy. This generates and maintains membrane lipid asymmetry, a property essential for a wide. As of june 2007, 400 unique and confirmed members of the p type atpase family have been discovered. Analysis of cisacting elements of p type atpase genes in chinese white pear. Structurally, all ptype atpases share certain common points. Fatpase, also known as ftype atpase, is an atp synthase found in bacterial plasma membranes, in mitochondrial inner membranes in oxidative phosphorylation, where it is known as complex v, and in. Four major domains are ubiquitously present in ptype atpases. F type atpase, proton transportes in mitochondria, synthesize atp 3. The alignment file for the p type atpase domain pf00690, pf00122 and pf246.
Methods and protocols provides comprehensive practical instructions for all researchers in the ptype atpase field, from the protein biochemist to the mouse geneticist, covering. We combine protein signatures from a number of member databases into a single. Arabidopsis thaliana has eight genes encoding members of the type 1b heavy metaltransporting subfamily of the ptype atpases. Functional properties of the coppertransporting atpase. There are many different classes of patpases, which. More than 150 members of the ptype atpase family have been identified. The 4 types of atp dependent transporters atpases f type. P4atpases comprise a family of ptype atpases that actively transport or flip phospholipids across cell membranes. A model of plant type iib and type iii atpases showing the. The ctpb gene encodes for a putative protein of 752 amino acids 77. The identification of genes that are involved in cd accumulationtolerance is vital in developing crop cultivars with low cd accumulation. Ptype atpases form a large superfamily of cation and lipid pumps.
Crystal structure of the plasma membrane proton pump nature. Pde1 encodes a ptype atpase involved in appressoriummediate plant infection by the rice blast fungus magnaporthe grisea. On the basis of the abovementioned results, it cannot be. Ptype atpases are a large family of membrane proteins that perform active ion transport across biological membranes. Rabbit sarcoplasmic reticulum caatpase is the only member of the ptype atpase family for which an atomic resolution structure is available 16. Rabbit sarcoplasmic reticulum ca atpase is the only member of the p type atpase family for which an atomic resolution structure is available 16. In these proteins, the energyproviding atp hydrolysis is coupled to ion. In plants and fungi, cellular ion homeostasis is powered by the proton pump, a member of the ptype atpase family. Specifically, p type atpases are active transporters of metal cations that are autophosphorylated on a. We developed a doubled haploid dh population that was derived. Structural biochemistrymembrane proteinsptype atpases. The vtype atpase is most prominently associated with lysosomes in mammals, but also appears to be expressed on the plasma membrane and neuronal synaptic vesicles. April 1995 esophageal, gastric, and duodenal disorders a165 identification of omeprazole response in dyspepsia. Ptype atpase in several rosaceae species including the pear have been identified zhang et al.
Drs2p is predicted to be a ptype atpase based on the presence of five wellconserved atpase motifs involved in the binding and hydrolysis of atp fig. Ptype atpases in health and disease frontiers research topic. Here we present functional studies and three new crystal structures of the rabbit skeletal. Pdf structurefunction relationship in ptype atpases a.
Rees1 division of chemistry and chemical engineering 11496. A novel p type atpase cloned from helicobacter pylori. In silico predictions show that ctpb belongs to the ptype. Ptype atpases are a large family of integral membrane transporters that are of vital importance in all. Ptype atpase was named based on the presence of an intermediate. Genomewide identification and analysis of ptype plasma. Pdf in the past 10 years, research on ptype atpases in plants has. Ptype atpases are found in all kingdoms of life and constitute a wide range of cation transporters, primarily for h, na, k, ca, and transition metal ions such as cui, znii, and cdii. The ptype atpase gene superfamily is one of the largest plant gene families. P type atpases undergo internal phosporhylation during transport cycle, i. The ion pumps of the ptype atpase family create electrochemical gradients that are essential for transepithelial transport, nutrient.
Ctpb is a plasma membrane copper i transporting ptype. P type atpases have a single catalytic subunit of 70 140 kda. Pdf ptype atpases are a large family of membrane proteins that perform active ion transport across biological. In plants, there are primarily three kinds of proton pumps, the vtype atpase, ptype atpase and ftype atpase. Pdf transient expression of ptype atpases in tobacco. Different ciselements are shown in different colors. These enzymes have a significant impact on cellular funciton. Download fulltext pdf download fulltext pdf download. Frontiers p4atpases as phospholipid flippasesstructure. The ptype atpase database was launched as part of a project performed at the traplabs, department of plant biology, the faculty of life sciences of the copenhagen university, copenhagen, denmark. A ptype atpase importer that discriminates between essential and toxic transition metals oded lewinson1, allen t. Comparative analysis of the ptype atpase gene family in. Current classification divides the ptype atpases into 5 subfamilies based on their sequence, cation. Purification and characterization of p type atpases.
A ptype atpase required for rice blast disease and. Interpro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. They are remarkably simple with only a single catalytic subunit and carry out large domain motions during transport. In general, the atpase enzymes are membrane proteins that perform energetically unfavorable processes, such as folding and degrading proteins, the initiation of replication, dna repair. In general, the atpase enzymes are membrane proteins that perform energetically unfavorable processes, such as folding and degrading proteins, the initiation of replication, dna repair, and the transport of substances, using the energy released during atp hydrolysis. It is also involved in plant resistance to a variety of biotic and abiotic factors, including salt. The transport activity of ptypeatpases is commonly inhibited by vanadate. As of june 2007, 400 unique and confirmed members of the ptype atpase family have been discovered. Ijms free fulltext hvpaa1 encodes a ptype atpase, a. The na,katpase or sodium pump carries out the coupled extrusion and uptake of na and k ions across the plasma membranes of cells of most higher eukaryotes. The 4 types of atpdependent transporters atpases ftype atp synthase synthesize atp hydrolyze atp ptype transporter. A ptype atpase importer that discriminates between.
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